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Test Code TCRB T-Cell Receptor V-Beta Repertoire Analysis, Spectratyping, Blood


Ordering Guidance


Mayo Clinic Laboratory Director/Consultant approval is required prior to ordering this test in patients greater than 40 years of age.



Additional Testing Requirements


Additional tests that could be ordered in conjunction with this test include:

-TRECS / T-Cell Receptor Excision Circles (TREC) Analysis, Blood

-CD4RT / CD4 T-Cell Recent Thymic Emigrants, Blood

-TCP / T-Cell Subsets, Naive, Memory, and Activated, Blood



Shipping Instructions


Specimens must be received in the laboratory on weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of collection.

 

Samples arriving over the weekend and/or on observed holidays may be canceled.



Necessary Information


Ordering physician's name and phone number are required.

 

TCR V beta Spectratyping Assay Patient Information (T719) is required. Testing will proceed without the form; however, results will be held under the information is received.



Specimen Required


For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.

 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume:

Adults: 10 mL

Pediatrics:

-Preferred volume for >1 year: 3 mL

-Preferred volume for ≤1 year: 1 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Type

Whole Blood EDTA

Specimen Minimum Volume

Adults: 5 mL
Pediatrics: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 48 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Clotted Reject

Reference Values

References values will be provided in the patient report.

Day(s) Performed

Varies

Report Available

20 to 24 days

Specimen Retention Time

Extracted DNA: 2 months

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81340-TRG (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, polymerase chain reaction)