Test Code TCRB T-Cell Receptor V-Beta Repertoire Analysis, Spectratyping, Blood
Ordering Guidance
Mayo Clinic Laboratory Director/Consultant approval is required prior to ordering this test in patients greater than 40 years of age.
Additional Testing Requirements
Additional tests that could be ordered in conjunction with this test include:
-TRECS / T-Cell Receptor Excision Circles (TREC) Analysis, Blood
-CD4RT / CD4 T-Cell Recent Thymic Emigrants, Blood
-TCP / T-Cell Subsets, Naive, Memory, and Activated, Blood
Shipping Instructions
Specimens must be received in the laboratory on weekdays and by 4 p.m. on Friday. Collect and package specimen as close to shipping time as possible.
It is recommended that specimens arrive within 24 hours of collection.
Samples arriving over the weekend and/or on observed holidays may be canceled.
Necessary Information
Ordering physician's name and phone number are required.
TCR V beta Spectratyping Assay Patient Information (T719) is required. Testing will proceed without the form; however, results will be held under the information is received.
Specimen Required
For serial monitoring, it is recommended to perform specimen collection at the same time of day, if possible.
Supplies: Ambient Shipping Box-Critical Specimens Only (T668)
Specimen Type: Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume:
Adults: 10 mL
Pediatrics:
-Preferred volume for >1 year: 3 mL
-Preferred volume for ≤1 year: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Type
Whole Blood EDTASpecimen Minimum Volume
Adults: 5 mL
Pediatrics: 1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Ambient | 48 hours | PURPLE OR PINK TOP/EDTA |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Clotted | Reject |
Reference Values
References values will be provided in the patient report.
Day(s) Performed
Varies
Report Available
20 to 24 daysSpecimen Retention Time
Extracted DNA: 2 monthsPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
81340-TRG (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, polymerase chain reaction)