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HelpTest Code NGPCM MayoComplete Plasma Cell Myeloma, Next-Generation Sequencing, Varies
Shipping Instructions
Bone marrow aspirate samples must arrive within 4 days of collection.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Bone marrow aspirate
Container/Tube: Lavender or pink top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collection Instructions:
1. Minimum plasma cell percentage is 5%.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
4. Label specimen as bone marrow.
5. Fresh specimen is required for this test, as testing is performed on sorted cells.
Specimen Stability Information: Ambient (preferred) 4 days/Refrigerate
Specimen Type: Paraffin-embedded bone marrow clot
Container/Tube: Paraffin block
Collection Instructions:
1. Send 1 slide stained with hematoxylin and eosin.
2. Minimum plasma cell percentage is 20%.
3. Required amount of tissue area is at least 25mm(2).
4. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.
5. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.
Specimen Stability Information: Ambient
Specimen Type: Tissue slide; bone marrow clot
Slides: 10 unstained slides
Container/ Tube: Transport in plastic slide holders.
Collection Instructions:
1. Send 10 unstained, nonbaked slides with 5-micron thick sections of tissue and 1 slide stained with hematoxylin and eosin
2. Minimum amount of plasma cells is 20%
3. Required amount of tissue area is at least 25mm(2).
4. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.
5. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.
Specimen Stability Information: Ambient
Genetics Test Information
This test includes next-generation sequencing to evaluate the following 26 genes and select intronic regions: BIRC3, BRAF, CCND1, CDKN2A, CRBN, CUL4A, CUL4B, CXCR4, DIS3, EGFR, IDH1, IDH2, IKZF1, IKZF3, KRAS, MYC, MYD88, NRAS, NSD2, PIK3CA, PIM1, STAT3, TENT5C, TP53, TRAF3, and XBP1.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CSPMM | NGPCM Pre-Analysis Cell Sorting, BM | No | No |
Specimen Type
VariesSpecimen Minimum Volume
Bone marrow aspirate: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Specimens that have been decalcified (all methods) Bone marrow core biopsies Paraffin shavings Frozen tissue Extracted DNA Moderately to severely clotted bone marrow aspirate |
Reject |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
16 to 21 daysSpecimen Retention Time
Bone marrow aspirate: 2 weeks; DNA: 3 months; FFPE tissue block: Unused portions of blocks will be returned to the client; Unstained slides: Not retainedPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81450