Ordering Guidance

This test should be performed on cases with a confirmed diagnosis of membranous nephropathy. Ideally, these cases should already be confirmed as phospholipase A2 receptor (PLA2R) negative, based on PLA2R immunohistochemistry (IHC) or immunofluorescence (IF) testing, not PLA2R serology testing.

If PLA2R IHC or IF testing has not been performed, order test PLAIF / Phospholipase A2 Receptor (PLA2R), Renal Biopsy and submit the additional required specimen.

Additional Testing Requirements

If phospholipase A2 receptor testing using immunohistochemistry or immunofluorescence (IF) has not previously been performed, PLAIF / Phospholipase A2 Receptor (PLA2R), Renal Biopsy must also be ordered and will be charged separately. An additional frozen IF block or frozen unstained slides will be required.

Necessary Information

The following information is required. Testing will not proceed without this information.

Note: Images can be submitted for the request via Mayo Clinic ImageShare (preferred) or external storage device. Be aware that ImageShare does not support the upload of Pathology whole slide images.

1. Preliminary pathology report and clinical history

2. Electron microscopy (EM) images

3. Immunofluorescence (IF) slides/images if EM images are not available.

Specimen Required

Specimen Type: Kidney tissue

Supplies: Pathology Packaging Kit (T554)

Container/Tube: Formalin-fixed, paraffin-embedded tissue block

Additional Information: Do not send fixed tissue slides. Testing can only be done on paraffin-embedded tissue blocks.