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Test Code MEV1 Methemoglobinemia Evaluation, Blood


Shipping Instructions


Specimen must arrive within 3 days (72 hours) of collection.



Necessary Information


Include recent transfusion information.

 

Include most recent complete blood cell count results.

 

Metabolic Hematology Patient Information (T810) is strongly recommended. Testing may proceed without this information, however if the information requested is received, any pertinent reported clinical features and data will drive the focus of the evaluation and be considered in the interpretation.

 

The laboratory has extensive experience in hemoglobin variant identification and many cases can be confidently classified without molecular testing. However, molecular confirmation is always available, subject to sufficient sample quantity (eg, multiplex ligation-dependent probe amplification testing requires at least 2 mL of sample in addition to protein testing requirements). If no molecular testing or specific molecular tests are desired, utilize the appropriate check boxes on the form. If the form or other communication is not received, the reviewing hematopathologist will select appropriate tests to sufficiently explain the protein findings, which may or may not include molecular testing.



Specimen Required


The following specimens are required for testing:

Whole blood ACD-B specimen

2 Whole blood EDTA specimens

 

Container/Tube: Lavender top (EDTA) and yellow top (ACD solution B)

Specimen Volume:

EDTA: Two 4-mL tubes

ACD: One 6-mL tube

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Reflex Tests

Test ID Reporting Name Available Separately Always Performed
SDEX Sickle Solubility, B Yes No
IEF Isoelectric Focusing, B No No
MASS Hb Variant by Mass Spec, B No No
UNHB Hb Stability, B No No
HPFH Hb F Distribution, B No No
ATHAL Alpha-Globin Gene Analysis Yes No
WASQR Alpha Globin Gene Sequencing, B Yes, (Order WASEQ) No
WBSQR Beta Globin Gene Sequencing, B Yes, (Order WBSEQ) No
WBDDR Beta Globin Cluster Locus Del/Dup,B Yes, (Order WBDD) No
WGSQR Gamma Globin Full Gene Sequencing Yes, (Order WGSEQ) No
MEV0 Methemoglobin Summary Interp No No

Specimen Type

Whole Blood ACD-B
Whole Blood EDTA

Specimen Minimum Volume

EDTA blood: 3 mL
ACD blood: 2.7 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 72 hours
Whole Blood EDTA Refrigerated 72 hours

Reject Due To

Gross hemolysis Reject

Reference Values

Definitive results and an interpretive report will be provided.

Specimen Retention Time

28 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83020-26-Hemoglobinopathy Interpretation

83020-Hb Variant, A2 and F Quantitation

83021-HPLC Hb Variant

82657-Methemoglobin reductase

83050-Methemoglobin, quantitative

83060-Sulfhemoglobin, quantitative

82664 (if appropriate)

83068 (if appropriate)

83789 (if appropriate)

88184 (if appropriate)

Day(s) Performed

Monday through Saturday

Report Available

3 to 25 days