Test Code MEV1 Methemoglobinemia Evaluation, Blood
Shipping Instructions
Specimen must arrive within 3 days (72 hours) of collection.
Necessary Information
Include recent transfusion information.
Include most recent complete blood cell count results.
Metabolic Hematology Patient Information (T810) is strongly recommended. Testing may proceed without this information, however if the information requested is received, any pertinent reported clinical features and data will drive the focus of the evaluation and be considered in the interpretation.
The laboratory has extensive experience in hemoglobin variant identification and many cases can be confidently classified without molecular testing. However, molecular confirmation is always available, subject to sufficient sample quantity (eg, multiplex ligation-dependent probe amplification testing requires at least 2 mL of sample in addition to protein testing requirements). If no molecular testing or specific molecular tests are desired, utilize the appropriate check boxes on the form. If the form or other communication is not received, the reviewing hematopathologist will select appropriate tests to sufficiently explain the protein findings, which may or may not include molecular testing.
Specimen Required
The following specimens are required for testing:
Whole blood ACD-B specimen
2 Whole blood EDTA specimens
Container/Tube: Lavender top (EDTA) and yellow top (ACD solution B)
Specimen Volume:
EDTA: Two 4-mL tubes
ACD: One 6-mL tube
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
SDEX | Sickle Solubility, B | Yes | No |
IEF | Isoelectric Focusing, B | No | No |
MASS | Hb Variant by Mass Spec, B | No | No |
UNHB | Hb Stability, B | No | No |
HPFH | Hb F Distribution, B | No | No |
ATHAL | Alpha-Globin Gene Analysis | Yes | No |
WASQR | Alpha Globin Gene Sequencing, B | Yes, (Order WASEQ) | No |
WBSQR | Beta Globin Gene Sequencing, B | Yes, (Order WBSEQ) | No |
WBDDR | Beta Globin Cluster Locus Del/Dup,B | Yes, (Order WBDD) | No |
WGSQR | Gamma Globin Full Gene Sequencing | Yes, (Order WGSEQ) | No |
MEV0 | Methemoglobin Summary Interp | No | No |
Specimen Type
Whole Blood ACD-BWhole Blood EDTA
Specimen Minimum Volume
EDTA blood: 3 mL
ACD blood: 2.7 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood ACD-B | Refrigerated | 72 hours | |
Whole Blood EDTA | Refrigerated | 72 hours |
Reject Due To
Gross hemolysis | Reject |
Reference Values
Definitive results and an interpretive report will be provided.
Specimen Retention Time
28 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
83020-26-Hemoglobinopathy Interpretation
83020-Hb Variant, A2 and F Quantitation
83021-HPLC Hb Variant
82657-Methemoglobin reductase
83050-Methemoglobin, quantitative
83060-Sulfhemoglobin, quantitative
82664 (if appropriate)
83068 (if appropriate)
83789 (if appropriate)
88184 (if appropriate)
Day(s) Performed
Monday through Saturday