Ordering Guidance

If testing directly from feces is desired, order HPFRP / Helicobacter pylori with Clarithromycin Resistance Prediction, Molecular Detection, PCR, Feces.

For more information see Helicobacter pylori Diagnostic Algorithm.

Specimen Required

The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Helicobacter pylori DNA is unlikely.

Submit only 1 of the following specimens:

Specimen Type: Fresh tissue or biopsy

Sources: Stomach (or duodenum)

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm (3) approximate size of a pencil eraser

Collection Instructions:

1. Collect fresh tissue specimen.

2. Submit tissue in a sterile container (without adding anything).

3. Refrigerate or freeze the specimen.

Specimen Stability Information: Refrigerated (preferred) 7 days/Frozen 7 days

Preferred:

Supplies: Tissue Block Container (T553)

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Sources: Stomach (or duodenum)

Container/Tube: Tissue block

Collection Instructions: Submit FFPE tissue block to be cut and returned.

Specimen Stability Information: Ambient (preferred)/Refrigerated

Acceptable:

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue scroll

Sources: Stomach (or duodenum)

Container/Tube: Sterile container for each individual cut section (scroll)

Collection Instructions: Perform microtomy and prepare five separate 10-micron sections. Each section (scroll) must be placed in a separate sterile container for submission.

Specimen Stability Information: Ambient (preferred)/Refrigerated