Ordering Guidance

This test is intended for instances when limited chronic eosinophilia fluorescence in situ hybridization (FISH) probes are needed. The FISH probes to be analyzed must be specified on the request, otherwise test processing may be delayed in order to determine the intended analysis. If specific probes are not included with this test request, this test may be canceled and automatically reordered by the laboratory as EOSDF / Chronic Eosinophilia, Diagnostic FISH, Varies.

If the entire chronic eosinophilia FISH panel is preferred, order EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies.

Paraffin embedded tissue testing is not available for these probe sets.

This test is ordered for targeted FISH probes to be evaluated based on specific abnormalities or on abnormalities identified in the diagnostic sample.

At diagnosis, a EOSDF / Chronic Eosinophilia, Diagnostic, FISH, Varies panel and a conventional chromosome study should be performed.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service.

Necessary Information

1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.

2. A reason for testing should be with each specimen. The laboratory will not reject testing if this information is not provided; however, appropriate testing and interpretation may be compromised or delayed. If not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.

3. A pathology and/or flow cytometry report may be requested by the laboratory to optimize testing and aid in interpretation of results.

Specimen Required

Submit only 1 of the following specimens:

Preferred

Specimen Type: Bone marrow

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 2-3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.

Acceptable

Specimen Type: Blood

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: Green top (heparin) or lavender top (EDTA)

Specimen Volume: 6 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.