Test Code EGFRW EGFR Targeted Mutation Analysis with ALK Reflex, Tumor
Necessary Information
A pathology report must accompany specimen for testing to be performed.
Specimen Required
Preferred: Submit both of the following specimens.
Acceptable: Submit at least one of the following specimens.
Specimen Type: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue.
Specimen Type: Tissue slide
Slides: 1 Hematoxylin and eosin-stained and 10 unstained
Collection Instructions:
Collection Instructions:
Submit the followings slides:
1 Slide stained with hematoxylin and eosin
AND
10 Unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.
Note: The total amount of required tumor nuclei can be obtained by scraping up to 10 slides from the same block.
Additional Information: Unused unstained slides will not be returned.
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
LCAF | ALK (2p23), Lung Cancer, FISH, Ts | Yes | No |
Specimen Type
VariesSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Varies | Ambient (preferred) | |
Refrigerated |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
5 to 14 daysSpecimen Retention Time
Tissue blocks: Unused portions of blocks will be returned; Tissue slides: Unused slides are stored for at least 5 yearsPerforming Laboratory

CPT Code Information
81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants(eg, exon 19 deletions, L858R, T790M, G719S, L861Q)
88381-Microdissection, manual
88271 x 2-DNA Probe (if appropriate)
88274-Interphase in situ hybridization (if appropriate)
88291-Interpretation and report (if appropriate)