Test Code DAT Direct Antiglobulin Test
Additional Codes
LAB274
Additional Information/Important Notes
Proper identification and specimen labelling is critical to safe blood transfusions as most hemolytic transfusion reactions result from errors in sample or patient identification.
- A blood bank banded specimen for possible transfusion can only be drawn by RN’s, phlebotomists, physicians, and lab techs that are employed at CPH. They must be at the hospital. Clinic and EMS draws for blood bank banded specimens will not be accepted. The 2nd verifier can be any CPH staff member who has been trained in proper patient identification.
- Two qualified persons must do the following together at the patient’s bedside or drawing station. Both parties are equally responsible for the accuracy of the information on the sample, and both will be held accountable for any errors.
Collection Method
Venipuncture
Minimum Volume
4ml
Container
6ml Purple Top EDTA
Acceptable Specimens
EDTA Whole Blood
Reference or Target Ranges
Negative
Reportable Units
Positive or Negative
Critical Value
Positive DAT for patients less than 1 year.
Reasons for Rejection
- Blood bank specimens are not like other specimens. The AABB Standard states that “the transfusion service shall accept only those samples that are completely, accurately, and legibly labeled.”
- The incorrect EPIC label is unacceptable: e.g. CBCD, CMP
- A patient chart label is not acceptable for a blood bank specimen.
- Failure to have two initials on the EPIC label will require a recollect.
- TWO labels on a blood bank banded specimen is unacceptable.
- The barcoded blood bank sticker and band cannot be added after the specimen has been sent down to the lab.
- Grossly hemolyzed specimens will be rejected.
- NOTE: If the time of collection is incorrect, the collector can come down to the lab and change it in the presence of the lab tech.
Specimen Stability
Ambient | 72 Hours |
Refrigerated (4°C to 8°C) | 72 Hours |
Frozen(-70°C to -20°C) | N/A |
Performance Information
Days and Time Performed | 24/7 |
Expected Turn Around Time |
Stat: within 60 minutes Routine: within 4 hours of arrival in lab |
Stat Availability | Yes |
Performing Bench | CPH Blood Bank |
Methodology/Method Description | Manual – Tube Method |
CPT Codes
86880
Last review/edit date
March 2024