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HelpTest Code CTB Mycobacteria and Nocardia Culture, Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesNecessary Information
1. Specimen source is required.
2. Alert the laboratory if Mycobacterium genavense is suspected, as this species requires addition of mycobactin J to the culture medium for optimal growth and recovery.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: 1.5 mL
Specimen Type: Bone marrow
Container/Tube: Sterile container, or green top (lithium or sodium heparin)
Specimen Volume: Entire collection
Specimen Type: Gastric washing
Container/Tube: Sterile container
Specimen Volume: 10 mL
Collection Instructions: Neutralize specimen within 4 hours of collection with 100 mg of sodium carbonate per 5 to 10 mL of gastric wash.
Specimen Type: Respiratory
Sources: Bronchoalveolar lavage fluid, bronchial washing, sputum
Container/Tube: Sterile container
Specimen Volume: 3 mL
Collection Instructions:
1. Collect 3 respiratory specimens for acid-fast smears and culture in patients with clinical and chest X-ray findings compatible with tuberculosis.
2. These 3 specimens should be collected at 8 to 24-hour intervals (24 hours when possible) and should include at least 1 first-morning specimen.
Specimen Type: Stool
Supplies: Stool Collection Kit, Random (T635)
Container/Tube: Sterile container
Specimen Volume: 5 to 10 g
Specimen Type: Tissue
Container/Tube: Sterile container
Specimen Volume: 5 to 10 mm
Collection Instructions: Collect a fresh tissue specimen.
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 20 to 50 mL
Collection Instructions: Collect a random urine specimen.
Fresh tissue or body fluid is the preferred specimen type instead of a swab specimen. Recovery of mycobacteria from swabs is generally very low yield.
Specimen Type: Swab
Sources: Wound, tissue, or body fluid
Container/Tube: Culture transport swab (noncharcoal) culturette, or Eswab
Specimen Volume: Adequate specimen
Collection Instructions:
1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.
2. Obtain secretions or fluid from source with sterile swab.
3. If smear and culture are requested or both a bacterial culture and mycobacterial culture are requested, collect a second swab to maximize test sensitivity.
Specimen will be rejected due to:
Blood or fixed tissue; specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth); swab sources of nasal, sinus, ear, mouth, throat, or scalp; wood shaft or charcoal swab
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Reference Values
Negative
Day(s) Performed
Monday through Sunday
CPT Code Information
87116-Mycobacterial Culture
87015-Mycobacteria Culture, Concentration (if appropriate)
87118-Id MALDI-TOF Mass Spec AFB (if appropriate)
87150-Id, Mtb Speciation, PCR (if appropriate)
87153-Mycobacteria Identification by Sequencing (if appropriate)
87176-Tissue Processing (if appropriate)
87150- Id, MTB complex Rapid PCR (if appropriate)
Report Available
42 to 70 daysReject Due To
| Blood or fixed tissue Specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) Saliva Swab sources of respiratory fluids (eg, sputum) Swab sources of nasal, sinus, ear, mouth, throat, or scalp Wood shaft or charcoal swab Petri dish |
Reject |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISMY | ID by 16S Sequencing | No, (Bill Only) | No |
| RMALM | Id MALDI-TOF Mass Spec AFB | No, (Bill Only) | No |
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | No |
| TBT | Concentration, Mycobacteria | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| LCTB | Id, MTB complex Rapid PCR | No, (Bill Only) | No |
Specimen Minimum Volume
See Specimen Required