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HelpTest Code CDGF Celiac Disease Gluten-Free Cascade, Serum and Whole Blood
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TTGA | Tissue Transglutaminase Ab, IgA, S | Yes | No |
| DAGL | Gliadin(Deamidated) Ab, IgA, S | Yes | No |
| DGGL | Gliadin(Deamidated) Ab, IgG, S | Yes | No |
| TTGG | Tissue Transglutaminase Ab, IgG, S | Yes | No |
| IGA | Immunoglobulin A (IgA), S | Yes | No |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumWhole Blood ACD-B
Ordering Guidance
This cascade should not be used in patients for whom human leukocyte antigen (HLA) DQ2/DQ8 typing has already been performed. For individuals who are positive for either DQ2 and/or DQ8, CDSP / Celiac Disease Serology Cascade, Serum should be ordered to assess for the presence of autoantibodies associated with celiac disease. For individuals who are negative for DQ2 and DQ8, no further testing is necessary as a diagnosis of celiac disease is unlikely.
Cascade testing is recommended for celiac disease. Cascade testing ensures that testing proceeds in an algorithmic fashion. The following cascades are available; select the appropriate one for your specific patient situation.
-CDCOM / Celiac Disease Comprehensive Cascade, Serum and Whole Blood: Complete testing including HLA DQ
-CDSP / Celiac Disease Serology Cascade, Serum: Complete serology testing excluding HLA DQ
-CDGF / Celiac Disease Gluten-Free Cascade, Serum and Whole Blood: For patients already adhering to a gluten-free diet
To order individual tests, see Celiac Disease Diagnostic Testing Algorithm
Specimen Required
Both whole blood and serum are required.
Specimen Type: Whole Blood
Container/Tube: Yellow top (ACD solution A or B)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood in original tube. Do not aliquot.
Specimen Type: Serum
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Specimen Minimum Volume
Blood: 3 mL
Serum: 1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days | ||
| Whole Blood ACD-B | Refrigerated (preferred) | ||
| Ambient | |||
Day(s) Performed
Profile tests: Monday through Friday; Reflex tests: Monday through Saturday
CPT Code Information
81376 x 2
82784 (if appropriate)
86258 (if appropriate)
86364 (if appropriate)
86231 (if appropriate)
Report Available
9 to 11 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
HLA-DQ TYPING
Presence of HLA-DQ2 or HLA-DQ8 alleles associated with celiac disease