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Test Code CATTR Cold Agglutinin Titer, Serum


Ordering Guidance


The cold agglutinin test is not specific for Mycoplasma pneumoniae and is not recommended to diagnose M pneumoniae infections. To diagnose M pneumoniae infections, order MPRP / Mycoplasmoides pneumoniae, Molecular Detection, PCR, Varies. For screening recent or past exposure to M pneumoniae, order MYCO / Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum.



Specimen Required


Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 4 mL

Pediatric Volume: 1 mL

Collections Instructions:

1. Use a warm pack to keep specimen at 37° C prior to and after collecting.

2. Allow specimen to clot at 37° C.

3. Centrifuge at 37° C and aliquot serum into plastic vial immediately after blood clots or within one hour of collection.

4. Do not refrigerate prior to separation of serum from red cells.


Specimen Type

Serum Red

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 7 days
  Frozen  7 days
  Ambient  72 hours

Reject Due To

Gross hemolysis OK
Gross icterus OK

Reference Values

Titer results:

>64: Elevated

>1000: May be indicative of hemolytic anemia

Day(s) Performed

Monday through Friday, Sunday

Report Available

1 to 3 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86157