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Test Code BLD CX PNL Blood Culture Pathogen Panel, NAAT

Additional Codes

LAB24337

Performing Laboratory

Central Peninsula Hospital

Collection Instructions

Optimal bottle fill volumes:

 

  • Bactec Aerobic bottle: 8 – 10 mL
  • Bactec Anaerobic bottle: 5 – 7 mL
  • Bactec Pediatric bottle: 1 – 3 mL

Collection Method

Follow blood culture collection procedure

Container

Set consisting of either:

  • Set of one aerobic Bactec bottle (Green) and one Lytic/10 Anaerobic Bactec bottle (Orange)

OR

  • one Pediatric Bactec bottle (yellow) only

 

Blood Culture Media Type Acceptable Manufacturer and Product Name
Aerobic

BD Bactec Plus Aerobic/F

BD Standard 10 Aerobic/F

bioMerieux BacT/ALERT FA Plus

bioMerieux BacT/ALERT SA

Anaerobic

BD Bactec Plus Anaerobic/F

BD Bactec Standard Anaerobic/F

BD Bactec Lytic/10 Aanaerobic/F

bioMerieux BacT/ALERT FN Plus

bioMerieux BacT/ALERT SN

Pediatric

BD Bactec Peds Plus/F

bioMerieux BacT/ALERT PF PLUS

Specimen Type

Whole blood in approved blood culture bottles

Minimum Volume

Bottle Type Minimum Volume
Aerobic standard (FA Plus) 5ml (a)
Anaerobic standard (FN Plus) 5ml (b)
Pediatric (PF Plus) 0.5ml

(a) If there is sufficient blood to fill one bottle only, fill the Aerobic bottle.

(b) Anaerobic bottles < 5mL may be accepted if accompanied by a standard Aerobic bottle containing at least 5 mL of blood.

 

Specimen Stability

Ambient 24 hours
Refrigerated (4°C to 8°C) N/A
Frozen(-40°C to 0°C) N/A

Reference or Target Ranges

Not Detected

Reportable Units

Detected or Not Detected

Critical Value

Detected

Reasons for Rejection

Hemolysis Threshold N/A
Icterus Threshold N/A
Lipemia Threshold N/A
Other QNS, contaminated, improperly labeled, improper specimen type, more than 3 sets in 24 hours, expired bottles

Performance Information

 

Days and Time Performed 24/7
Expected Turn Around Time Stat: 1 hour
Routine: 4 hours
Stat Availability Yes
Performing Bench CPH Microbiology
Methodology/Method Description bioMérieux BioFire FilmArray Torch 

Panel Components

BioFire FilmArray BCID2 Targets


Gram Positive Bacteria

Enterococcus faecalis, Enterococcus faecium, Listeria monocytogenes, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lugdunensis, Streptococcus agalactiae (Group B),  Streptococcus pneumonia, Streptococcus pyogenes (Group A)

 

Gram Negative Bacteria

Acinetobacter calcoaceticus-baumannii complex, Bacteroides fragilis, Haemophilus influenza, Neisseria meningitidis (encapsulated), Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Enterobacter cloacae complex, Klebsiella aerogenes, Klebsiella oxytoca, Klebsiella pneumoniae group, Proteus spp, Salmonella spp, Serratia marcescens

 

Yeast

Candida albicans, Candida auris, Candida glabrata, Candida kruse, Candida parapsilosis, Candida tropicalis, Cryptococcus neoformans/gatii

 

Antimicrobial Resistance Genes

CTX-M, IMP, KPC, Mcr-1, mecA/C, mecA/C and MREJ (MRSA), NDM, OXA-48-lik,e vanA/B, VIM

CPT Codes

87154

Last review/edit date

2025