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Test Code BCGRV Immunoglobulin Gene Rearrangement, PCR, Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


Body fluid or spinal fluid must arrive within 4 days of collection.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Paraffin-embedded tissue

Container/Tube: Paraffin block

Collection Instructions:

1. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

2. Indicate specimen source.

3. Include pathology report.

Specimen Stability Information: Ambient

Additional Information: If the quality of the biopsy specimen is poor, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.

 

Acceptable:

Specimen Type: Tissue slide

Slides: 20 unstained slides

Container/ Tube: Transport in plastic slide holders.

Collection Instructions:

1. Send 20 unstained, nonbaked slides with 5-micron thick sections of tissue.

2. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.

3. Indicate specimen source.

4. Include pathology report.

Specimen Stability Information: Ambient

Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.

 

Specimen Type: Body fluid

Sources: Pleural, peritoneal, vitreous, and spinal fluid (CSF)

Container/Tube: Sterile container

Specimen Volume: At least 5 mL

Collection Instructions:

1. If the volume is large, pellet cells prior to sending.

2. Send less volume at ambient temperature or as a frozen cell pellet.

3. Specify the type of fluid being submitted.

Specimen Stability Information:

Body fluid: Ambient 4 days/Refrigerated/Frozen

Cell pellet: Frozen

 

Specimen Type: Frozen tissue

Container/Tube: Plastic container

Specimen Volume: 100 mg

Collection Instructions:

1. Freeze tissue within 1 hour of collection.

2. Indicate specimen source.

Specimen Stability Information: Frozen

 

Specimen Type: Extracted DNA

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted within 7 days after collection.

2. Label specimen as extracted DNA and source of specimen.

3. Provide volume and concentration of DNA.

Specimen Stability Information: Frozen (preferred)/Refrigerated/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.


Specimen Minimum Volume

Body Fluid: 1 mL; Frozen tissue: 50 mg; Extracted DNA: 50 microliters (mcL) at 20 ng/mcL; Tissue slides: 10 unstained slides

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

CPT Code Information

81261-IGH (Immunoglobulin heavy chain locus) (eg, leukemias and lymphomas B-cell), gene rearrangement analysis to detect abnormal clonal populations; amplified methodology (eg. polymerase chain reaction)

81264-IGK (Immunoglobulin kappa light chain locus) (eg, leukemia and lymphoma, B-cell) gene rearrangement analysis, evaluation to detect abnormal clonal populations

Report Available

7 to 14 days

Specimen Retention Time

Fresh/Frozen Tissue: 2 weeks; Extracted DNA: 3 months; FFPE tissue: Unused portions of blocks will be returned to the client. Unstained slides/body fluid: Not retained

Reject Due To

Bone marrow core biopsies Reject
Paraffin shavings Reject