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Test Code ANST Androstenedione, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Ordering Guidance


 



Additional Testing Requirements


For diagnosis and differential diagnosis of hyperandrogenism, an initial workup in adults should also include total and bioavailable testosterone (TTBS / Testosterone, Total and Bioavailable, Serum) measurements. Depending on results, this may be supplemented with measurements of sex hormone-binding globulin (SHBG1 / Sex Hormone-Binding Globulin, Serum) and other androgenic steroids (eg, dehydroepiandrosterone sulfate [DHEA-S]).

 

For diagnosis of congenital adrenal hyperplasia (CAH), the following assays should also be ordered:

-OHPG / 17-Hydroxyprogesterone, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-CORT / Cortisol, Serum

 

For monitoring CAH treatment, the following assays should also be ordered:

-TTST / Testosterone, Total, Mass Spectrometry, Serum

-OHPG / 17-Hydroxyprogesterone, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-DHEA_ / Dehydroepiandrosterone [DHEA], Serum.

 

For diagnosis of premature adrenarche, the following assays should also be ordered:

-FSH / Follicle-Stimulating Hormone [FSH], Serum

-LH / Luteinizing Hormone [LH], Serum

-TTBS / Testosterone, Total and Bioavailable, Serum or TGRP / Testosterone, Total and Free, Serum

-EEST / Estradiol, Serum

-DHES1 / Dehydroepiandrosterone Sulfate, Serum

-DHEA_ / Dehydroepiandrosterone (DHEA), Serum

-SHBG1 / Sex Hormone-Binding Globulin, Serum

-OHPG / 17-Hydroxyprogesterone, Serum



Specimen Required


Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  7 days

Reference Values

PEDIATRICS*

 Infants

Age

Reference range (ng/dL)

Premature: 26-28 weeks

4 days

92-282 ng/dL

Premature: 31-35 weeks

4 days

80-446 ng/dL

Full term

1-7 days

20-290 ng/dL

Full term

1 month-1 year

<69 ng/dL

 

 

*Male Tanner stages

Age (Years)

Reference range (ng/dL)

Stage I (prepubertal)

<9.8

<51

Stage II

9.8-14.5

31-65

Stage III

10.7-15.4

50-100

Stage IV

11.8-16.2

48-140

Stage V

12.8-17.3

65-210

 

 

*Female Tanner stages

Age (Years)

Reference range (ng/dL)

Stage I (prepubertal)

<9.2

<51

Stage II

9.2-13.7

42-100

Stage III

10.0-14.4

80-190

Stage IV

10.7-15.6

77-225

Stage V

11.8-18.6

80-240

 

*Soldin SJ, Brugnara C, Wong EC, eds. Androstenedione. In: Pediatric Reference Ranges. 4th ed. AACC Press; 2003: 32-34

 

ADULTS

Males: 40-150 ng/dL

Females: 30-200 ng/dL

 

For SI unit Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) Performed

Monday through Friday

CPT Code Information

82157

Report Available

2 to 5 days

Specimen Retention Time

14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK