Test Code ALLOI Allo-isoleucine, Blood Spot
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole bloodSpecimen Required
Supplies:
-Card-Blood Spot Collection (Filter Paper) (T493)
-Card-Postmortem Screening (Filter Paper) (T525)
Container/Tube:
Preferred: Blood Spot Collection (Filter Paper)
Acceptable: Local newborn screening card, Whatman Protein Saver 903 filter paper, PerkinElmer 226 filter paper, Munktell filter paper, Postmortem Screening Card
Specimen Volume: 2 Blood spots
Collection Instructions:
1. Do not use device or capillary tube containing EDTA to collect specimen.
2. At least 1 spot should be complete and unpunched.
3. An alternative blood collection option for a patient older than 1 year is a fingerstick. For detailed instructions, see How to Collect a Dried Blood Spot Sample.
4. Include type of feeding information on the collection card.
5. Let blood dry on filter paper at ambient temperature in a horizontal position for a minimum of 3 hours.
6. Do not expose specimen to heat or direct sunlight.
7. Do not stack wet specimens.
8. Keep specimen dry.
Additional Information:
1. For collection instructions see Blood Spot Collection Instructions.
2. For collection instructions in Spanish, see Blood Spot Collection Card-Spanish Instructions (T777).
3. For collection instructions in Chinese, see Blood Spot Collection Card-Chinese Instructions (T800).
Specimen Minimum Volume
1 Blood spot
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole blood | Ambient (preferred) | 59 days | FILTER PAPER |
| Frozen | 59 days | FILTER PAPER | |
| Refrigerated | 59 days | FILTER PAPER |
Reference Values
Allo-isoleucine: <4 nmol/mL
Leucine: 52-269 nmol/mL
Isoleucine: 22-167 nmol/mL
Valine: 84-414 nmol/mL
An interpretive report will also be provided.
Day(s) Performed
Monday through Friday
CPT Code Information
82136
Genetics Test Information
Second-tier test for abnormal newborn screen and follow-up of patients with maple syrup urine disease.
Report Available
2 to 4 daysSpecimen Retention Time
1 yearReject Due To
| Blood spot specimen that shows layering | Reject |
| Blood spot specimen that shows serum rings | Reject |
| Blood spot specimen that shows multiple applications | Reject |
| Blood spot specimen that shows insufficient specimen | Reject |