Test Code SFEC Iron and Total Iron-Binding Capacity, Serum
Ordering Guidance
The recommended primary test for assessment of iron deficiency is serum ferritin. Order FERR1 / Ferritin, Serum.
Additional Testing Requirements
Although measurement of serum iron, total iron-binding capacity, and percent saturation should not be used as primary testing for iron deficiency, they may be helpful when used in conjunction with ferritin and soluble transferrin receptor, especially in patients with inflammation. Order both FERR1 / Ferritin, Serum; and STFR / Soluble Transferrin Receptor (sTfR), Serum with this test.
Specimen Required
Patient Preparation:
1. Fasting (12 hours)
2. For 24 hours before collection, patient should not take iron-containing supplements.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Draw blood before noon (preferred).
2. Within 2 hours of collection, serum gel tubes should be centrifuged.
3. Within 2 hours of collection, red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial.
Specimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 7 days | |
Frozen | 180 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
IRON
Males: 50-150 mcg/dL
Females: 35-145 mcg/dL
TOTAL IRON-BINDING CAPACITY
250-400 mcg/dL
PERCENT SATURATION
14-50%
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 2 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
83540-Iron
83550-Iron-binding capacity