Test Code LCMS Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
FCINT | Flow Cytometry Interp, 2-8 Markers | No, (Bill Only) | No |
FCIMS | Flow Cytometry Interp, 9-15 Markers | No, (Bill Only) | No |
FCINS | Flow Cytometry Interp,16 or greater | No, (Bill Only) | No |
AMLMF | AML, Specified FISH | Yes | No |
AMLAF | Adult AML, FISH | Yes | No |
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
VariesOrdering Guidance
For B-cell acute lymphoblastic leukemia minimal residual disease testing in either blood or bone marrow, order BALLM /Â B-Cell Lymphoblastic Leukemia Monitoring, Minimal Residual Disease Detection, Flow Cytometry, Varies.
This test is appropriate for hematopoietic specimens only. For solid tissue specimens, order LLPT / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Tissue.
For bone marrow specimens being evaluated for possible involvement by a myelodysplastic syndrome (MDS) or a myelodysplastic/myeloproliferative neoplasm (MDS/MPN) including chronic myelomonocytic leukemia (CMML), order MYEFL / Myelodysplastic Syndrome by Flow Cytometry, Bone Marrow.
Bronchoalveolar lavage specimens submitted for evaluation for leukemia or lymphoma are appropriate to send for this test.
This test is not appropriate for and cannot support diagnosis of sarcoidosis, hypersensitivity pneumonitis, interstitial lung diseases, or differentiating between pulmonary tuberculosis and sarcoidosis (requests for CD4/CD8 ratios); specimens sent for these purposes will be rejected.
This test is not intended for product of conception (POC) specimens. For POC specimens see CMAPC / Chromosomal Microarray, Autopsy, Products of Conception, or Stillbirth.
Additional Testing Requirements
For bone marrow testing, if cytogenetic tests are desired along with this test request, an additional specimen should be submitted. It is important that the specimen be obtained, processed, and transported according to instructions for the other test.
Shipping Instructions
Specimen must arrive within 4 days of collection.
Necessary Information
The following information is required:
1. Pertinent clinical history including reason for testing or clinical indication/morphologic suspicion.
2. Specimen source
3. For spinal fluid specimens: spinal fluid cell and differential counts are required
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or green top (sodium heparin)
Specimen Volume: 6 mL
Slides: If possible, include 5 to 10 unstained blood smears labeled with two unique identifiers
Collection Instructions:
1. Send whole blood specimen in original tube. Do not aliquot.
2. Label specimen as blood.
Specimen Stability Information: Ambient 4 days/Refrigerated 4 days
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD solution A or B)
Acceptable: Lavender top (EDTA) or green top (sodium heparin)
Specimen Volume: 1 to 5 mL
Slides: If possible, include 5 to 10 unstained bone marrow aspirate smears, which must be labeled with two unique identifiers.
Collection Instructions:
1. Submission of bilateral specimens is not required.
2. Send bone marrow specimen in original tube. Do not aliquot.
3. Label specimen as bone marrow.
Specimen Stability Information: Ambient 4 days/Refrigerated 4 days
Note: A fresh (less than 4 days post-collection), unfixed, non-embedded bone marrow core biopsy, bone or bone lesion is acceptable as an equivalent source for bone marrow aspirate for this test only in the event of a dry tap during the bone marrow harvesting procedure. Indicate "dry tap" in performing lab notes or paperwork when submitting this specimen type.
Specimen Type: Fluid
Sources: Serous effusions, pleural fluid, pericardial fluid, abdominal (peritoneal) fluid
Container/Tube: Body fluid container
Specimen Volume: 20 mL
Collection Instructions:
1. If possible, fluids other than spinal fluid should be anticoagulated with heparin (1 U/mL of fluid).
2. Label specimen with fluid type.
Specimen Stability Information: Refrigerated 4 days/Ambient 4 days
Additional Information: The volume of fluid necessary to phenotype the lymphocytes or blasts in serous effusions depends upon the cell count in the specimen. Usually, 20 mL of pleural or peritoneal fluid is sufficient. Smaller volumes can be used if there is a high cell count.
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 1 to 1.5 mL
Collection Instructions:
1. An original cytospin preparation (preferably unstained) should be included with the spinal fluid specimen so correlative morphologic evaluation can occur.
2. Label specimen as spinal fluid.
Specimen Stability Information: Refrigerated 4 days/Ambient 4 days
Additional Information: The volume of fluid necessary to phenotype the lymphocytes or blasts in spinal fluid depends upon the cell count in the specimen. A cell count should be determined and submitted with the specimen. Usually 1 to 1.5 mL of spinal fluid is sufficient. Smaller volumes can be used if there is a high cell count. If cell count is less than 10 cells/mcL, a larger volume of spinal fluid may be required. When cell counts drop below 5 cells/mcL, the immunophenotypic analysis may not be successful.
Specimen Minimum Volume
Blood: 3 mL
Bone marrow: 0.5mL
Spinal fluid: 1 mL
Fluid from serous effusions: 5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Varies |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
CPT Code Information
88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1
88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each)
88187-Flow Cytometry Interpretation, 2 to 8 Markers (if appropriate)
88188-Flow Cytometry Interpretation, 9 to 15 Markers (if appropriate)
88189-Flow Cytometry Interpretation, 16 or More Markers (if appropriate)
Report Available
1 to 4 daysSpecimen Retention Time
Remaining blood/bone marrow:14 days; Remaining fluid, 7 daysReject Due To
Gross hemolysis | Reject |
Fully clotted whole blood | Reject |