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Test Code FRAG Osmotic Fragility, Erythrocytes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Control
Whole Blood EDTA


Ordering Guidance


 



Additional Testing Requirements


 



Shipping Instructions


Specimens must arrive within 72 hours of collection.



Necessary Information


Patient's age is required.



Specimen Required


Both a whole blood EDTA specimen and a shipping control specimen are required. The shipping control specimen is used to evaluate whether a patient result has been compromised by handling conditions such as temperature, motion, or other transportation interferences. Temperature and handling extremes can adversely impact the integrity of the specimen.

 

Patient:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Refrigerate specimen immediately after collection.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together.

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Collect a shipping control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Clearly hand write “normal control” on the outermost label.

3. Refrigerate specimen immediately after collection.

4. Send control specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. The control and patient specimen must be handled in the same manner from specimen collection to receipt in the testing laboratory.


Specimen Minimum Volume

Patient whole blood, shipping control: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Control Refrigerated 72 hours PURPLE OR PINK TOP/EDTA
Whole Blood EDTA Refrigerated 72 hours

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

NaCl = sodium chloride

Reference values have not been established for patients who are younger than 12 months of age.

Day(s) Performed

Monday through Saturday

CPT Code Information

85557

Report Available

2 to 5 days

Specimen Retention Time

7 days

Reject Due To

Gross hemolysis Reject
Clotted blood Reject