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HelpTest Code FGEN Fungal Culture, Routine
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
Nocardia and the other aerobic actinomycetes are not fungi, therefore a fungal culture should not be ordered. However, these organisms grow well on mycobacterial medium. When infection with this group of organisms is suspected, order CTB / Mycobacteria and Nocardia Culture, Varies.
Shipping Instructions
Specimen should arrive within 24 hours of collection.
Necessary Information
Specimen source (anatomic body site) is required.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Fresh tissue
Container/Tube: Sterile container
Specimen Volume: Pea size
Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.
Acceptable:
Specimen Type: Bone marrow
Container/Tube: Sterile container, SPS/Isolator system, or green top (lithium or sodium heparin)
Specimen Volume: Entire collection
Specimen Type: Respiratory specimen
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions: Collect a random urine specimen.
Fresh tissue or body fluid are preferred over a swab specimen. Recovery of mycobacteria from swabs is generally very low yield. Only submit a swab specimen if tissue biopsy or fluid aspiration is not possible.
Specimen Type: Swab
Supplies: BD E-Swab (T853)
Sources: Dermal, ear, mouth, ocular, throat, or wound
Container/Tube: Sterile, screw-capped tube containing Liquid Amies Medium with flocked swab (eg, E-Swab)
Specimen Volume: 1 mL in swab container with swab
Collection Instructions:
1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.
2. Obtain secretions or fluid from source with sterile flocked swab. Paranasal sinus collections must use a nasopharyngeal flocked swab.
3. Place flocked swab in sterile, screw-capped tube containing 1 mL of Liquid Amies Medium.
4. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity. Submit each swab in a separate sterile, screw-capped tube with 1 mL of Liquid Amies Medium.
Specimen Minimum Volume
Bone marrow or body fluid: 1 mL; Cerebrospinal fluid: 0.5 mL; Respiratory specimen: 1.5 mL; All other specimen types: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Reference Values
Negative
If positive, fungus will be identified.
Day(s) Performed
Monday through Sunday
CPT Code Information
87102-Fungal culture, routine
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87153-ITS Fungal Sequencing (if appropriate)
87176-Tissue processing (if appropriate)
Report Available
24 to 35 daysSpecimen Retention Time
Raw specimen: 7 daysReject Due To
| Blood or fixed tissue | Reject |
| Specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) | Reject |
| Swab sources of respiratory fluids (eg, sputum) or nasal swab | Reject |
| Wood shaft or charcoal swab | Reject |
| Catheter tips | Reject |
| Petri dish | Reject |
| Stool | Reject |
| Blades from scalpels or razors | Reject |
| Boric acid tubes | Reject |
| Aptima swab | Reject |
| Culture transport swabs (eg, Culturette) | Reject |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| ITSF | ITS Fungal Sequencing | No, (Bill Only) | No |