Specimen Required

Container/Tube: Unstained cytospin slide

Specimen Volume: 1 slide

Collection Instructions:

1. Collect a random urine specimen.

2. Centrifuge 10 mL of a random urine collection.

3. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBC are seen, the eosinophil test cannot be performed. If WBC are present, prepare slides using the centrifuged sediment.

4. The unstained slide should be prepared within 2 hours of collection. Only an unstained slide will be accepted. Any other specimen, including the raw urine specimen, will be rejected.

5. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.

6. Slides need to be labeled with patient name and date of birth

7. Guidelines for slide preparation. Use the sediment to prepare slides as follows:

a. 150 mcL of sample for 1 to 3 WBC/high power field (hpf)

b. 100 mcL of sample for moderate to normal sediment (<50 cell/hpf)

c. 50 mcL of sample for heavier sediment (>50 cells/hpf)

d. 25 mcL for packed fields of sediment